Volatiles from Liquid Cultures of Lentinellus cochleatus (Basidiomycotina)

Hans-Peter Hanssen Lehrstuhl für Pharmakognosie, Bundesstraße 43, D-2000 Hamburg 13, Bundesrepublik Deutschland and Wolf-Rainer Abraham Gesellschaft für Biotechnologische Forschung mbH (G BF), Mascheroder Weg 1, D-3300 Braunschweig, Bundesrepublik Deutschland Z. Naturforsch. 41c, 959—962 (1986); received July 7, 1986 Lentinellus cochleatus, Basidiomycotina, Liquid Cultures, Fungal Volatiles, Fungal Terpenoids The basidiomycete Lentinellus cochleatus CBS 201.47 was cultivated on a defined synthetic liquid culture medium containing glucose (2% ), asparagine (0.15% ), malt extract (0.2% ), and mineral salts. 12 weeks old cultures produced a “sw eet” odour with an aniseor cinnamon-like note. 3 major volatiles could be identified from the steam distillates by their GC/MSand ’H NMR data. Predominant constituents were the acyclic sesquiterpene alcohols trans-nerolidol (41.4% ) and fokienol (14.8% ). 2,2-Dimethyl-6-formylchromene (6.1% ) is described for the first time as a natural product. Besides these, 15 minor constituents could be identified or chemically charac­ terized, respectively, the majority of them being sesquiterpene hydrocarbons and alcohols.